STUDY OF THE INFLUENCE OF CALCIUM ANTAGONISTS ON THE LEVEL OF LUTEINIZING HORMONE IN THE BLOOD PLASMA

Abstract

The purpose of the study was to study the changes in the level of luteinizing hormone in the blood against the background of long-term intake of calcium antagonists. Materials and methods. The research was carried out on 82 male white rats weighing 180-230 grams, each of which reached sexual maturity. Appropriate representatives of calcium antagonists were administered to the animals in the experiment: verapamil 5, 25 mg/kg ("Izoptin" Abbot Laboratories S.A., Italy), nifedipine 5, 10 mg/kg ("Farmadipin" Farmak Ukraine), diltiazem 5, 20 mg/kg ("Diltizem - L", MNIS- Istanbul) was used. The concentration of luteinizing hormone in the blood plasma was determined by the enzyme immunoassay method in the "BioScreen MS-500" device. For this purpose, blood was taken from the heart of experimental animals given ether narcosis. "Elisa kit LH" reagent kit was used to determine the concentration of luteinizing hormone in the blood. Results. The study showed that the concentration of luteinizing hormone in the blood of female rats varies depending on the dose. The concentration of luteinizing hormone in the blood plasma of animals injected with 25 mg/kg dose of verapamil and 20 mg/kg dose of diltiazem was sharply decreased. The concentration of luteinizing hormone in the blood plasma of animals injected with nifedipine at a dose of 10 mg/kg, verapamil 5 mg/kg and diltiazem 5 mg/kg decreased less than the concentration of the corresponding hormone in the blood plasma of injected animals. Conclusion. It can be said that the use of 5 mg/kg and 10 mg/kg doses of nifedipine is more appropriate for the pharmacotherapy of a number of pathologies where the use of KA is necessary in the reproductive age, compared to other representatives of the relevant drug group.