STUDY OF ACUTE TOXİCİTY OF BİOLOGİCALLY ACTİVE MİXTURES OBTAİNED FROM МЕLISSA (MELİSSA OFFİCİNALİS) AND COMMON HOP (HUMULUS LUPULUS)

Abstract

The article is devoted to the study of acute toxicity of biologically active mixtures obtained from rosemary and St. John's wort. For this purpose, biologically active mixtures obtained from both plants were administered to white rats (males and females) weighing 170-200 g and white mice weighing 16-22 g, starting with 50 mg/kg and ending at 1000 mg/kg per body weight intraperitoneally . Each group had 5 males and 5 females. Within 72 hours, the condition of the animals, behavioral characteristics, intensity and nature of their motor activity, number and nature of seizures, coordination of behavior, skeletal muscle tone, depth and frequency of respiratory movements, rhythm of heartbeats, condition of fur and skin, color of mucous membranes, pupil size were assessed. ,condition of the tail, consistency and amount of feces, frequency of urination, color of urine, need for water and food, reaction to tactile, sound and light stimuli were studied visually. As a result of our research, we came to the conclusion that biologically active mixtures obtained from melissa and common hop wort plants did not cause death in experimental animals in the dose range from 50 mg/kg to 1000 mg/kg, so it was impossible to determine LD-50. This can be explained by the weak toxic effect of biologically active mixtures obtained from these plants.