Morphological characteristics changes structure and permeability of gubernaculum vessels in the cryptorchidism. Light and electronic microscopic research

Abstract

Aim. Currently, the purpose of the research is to investigate the changes in gubernaculum vessels at light and electron microscopic levels during cryptorchidism compared to the control group.

Material end methods. From araldite-epon blocks of the gubernaculum biopsy materials taken from 12 patients the semithin (1-2 μm) and ultrathin (35-70 nm) sections were obtained by means of ultramicrotome Leica EM UC7. Semi-thin sections (1-2 μm) were stained by methylene blue, azure II and fuchsin. Examination of ultrathin sections (50-70 nm) was performed on electron microscope JEM-1400 (Japan) at an accelerating voltage of 80-100 kV.

Results. Blood stasis as a result of obturation of muscular venules caused by their thrombosis; open contacts between endothelial cells in the newly developed collateral vessels; fenestrations formed in peripheral parts of endothelial cells as a result of their contraction; transendothelial canals formed by fusion of caveoles taken together lead to the accumulation of edema fluid inside the gubernaculums in cryptorchidism.

Conclusion. High levels of filtration in the microvasculature vessels for a long time can increase the volume of gubernaculum to such a level that its passage together with the testes through the inguinal canal may become impossible.