Electronmicroscopic research of endothelium of vessels in modeling anthracycline cardiomyopathy

Abstract

The purpose of the study. Study of the electron-microscopic reorganization of the vascular endothelium of the myocardial microvasculature in the simulation of acute, subacute and chronic forms of anthracycline cardiomyopathy.

Material and  metods. The object of the study was the myocardium of 30 experimental rats in which, by intraperitoneal administration of the antibiotic doxorubicin in a cumulative dose of 15 mg / kg, acute (n = 10), subacute (n = 10) and the chronic form (n = 10) of anthracycline cardiomyopathy were modulated. In  the investigation the  electronic microscopic method  is used. The material was processed according to the standard technique. Sections were prepared on an LKB ultratome (Sweden) and viewed in a JEM-100B electron microscope (Japan).

Results of research. After the development of acute anthracycline cardiotoxicity, damage and destruction of the basement membranes of the endothelial lining and endothelial organelles, hypertrophy and edema of endothelial cells are detected. The subacute form of anthracycline cardiomyopathy is characterized by the progression of the processes of alteration of the vessels, accompanied by the development of necrosis of endotheliocytes, leading to cell death and destruction of the vessels. The development of chronic anthracycline cardiomyopathy leads to endothelial cell apoptosis and destruction of the vessels of the myocardial microvasculature, which leads to myocardial ischemia.